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primescript ii high fidelity rt pcr  (TaKaRa)


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    Structured Review

    TaKaRa primescript ii high fidelity rt pcr
    Primescript Ii High Fidelity Rt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+high+fidelity+rt+pcr+kit/PrimeScript+High+Fidelity+RT-PCR+Kit/pmc13155309-177-17-28
    Average 96 stars, based on 515 article reviews
    primescript ii high fidelity rt pcr - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Synthesized:

    Article Title: Anti-Tumor Effects of Cecropin A and Drosocin Incorporated into Macrophage-like Cells Against Hematopoietic Tumors in Drosophila mxc Mutants
    Article Snippet: Total RNA was extracted from 14 to 18 pairs of FB of mature third-instar larvae using Trizol Regent® (Invitrogen, Waltham, MA, USA). .. After treatment with DNase I (Epicentre Technologies, Madison, WI, USA) to remove mixed genomic DNA, the purity of the RNA was checked by ensuring that the A260–A280 ratio of each RNA sample was between 1.8 and 2.0. cDNA was synthesized from the total RNA using a PrimeScript High-Fidelity RT-PCR Kit (TaKaRa, Clontech Laboratories, Shiga, Japan). .. Real-time PCR reactions were performed on a Thermal Cycler Dice® Real-Time System III (TaKaRa Bio, Shiga, Japan) using TB Green® Premix Ex TaqTM II Tli RNaseH Plus (TaKaRa Bio, Shiga, Japan).

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. After viral RNA purification, cDNA from viral mRNA was synthesized by reverse transcription reaction with oligo dT primer using PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio, Cat. #R022A). .. The amount of N mRNA was quantified by qPCR using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Cat. # QPX-201).

    Article Title: Anti-Tumor Effects of Cecropin A and Drosocin Incorporated into Macrophage-like Cells Against Hematopoietic Tumors in Drosophila mxc Mutants
    Article Snippet: Total RNA was extracted from 14 to 18 pairs of FB of mature third-instar larvae using Trizol Regent ® (Invitrogen, Waltham, MA, USA). .. After treatment with DNase I (Epicentre Technologies, Madison, WI, USA) to remove mixed genomic DNA, the purity of the RNA was checked by ensuring that the A260–A280 ratio of each RNA sample was between 1.8 and 2.0. cDNA was synthesized from the total RNA using a PrimeScript High-Fidelity RT-PCR Kit (TaKaRa, Clontech Laboratories, Shiga, Japan). .. Real-time PCR reactions were performed on a Thermal Cycler Dice ® Real-Time System III (TaKaRa Bio, Shiga, Japan) using TB Green ® Premix Ex TaqTM II Tli RNaseH Plus (TaKaRa Bio, Shiga, Japan).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Anti-Tumor Effects of Cecropin A and Drosocin Incorporated into Macrophage-like Cells Against Hematopoietic Tumors in Drosophila mxc Mutants
    Article Snippet: Total RNA was extracted from 14 to 18 pairs of FB of mature third-instar larvae using Trizol Regent® (Invitrogen, Waltham, MA, USA). .. After treatment with DNase I (Epicentre Technologies, Madison, WI, USA) to remove mixed genomic DNA, the purity of the RNA was checked by ensuring that the A260–A280 ratio of each RNA sample was between 1.8 and 2.0. cDNA was synthesized from the total RNA using a PrimeScript High-Fidelity RT-PCR Kit (TaKaRa, Clontech Laboratories, Shiga, Japan). .. Real-time PCR reactions were performed on a Thermal Cycler Dice® Real-Time System III (TaKaRa Bio, Shiga, Japan) using TB Green® Premix Ex TaqTM II Tli RNaseH Plus (TaKaRa Bio, Shiga, Japan).

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. After viral RNA purification, cDNA from viral mRNA was synthesized by reverse transcription reaction with oligo dT primer using PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio, Cat. #R022A). .. The amount of N mRNA was quantified by qPCR using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Cat. # QPX-201).

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. RT-PCR was performed with the PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio) using these strand-specific primers to synthesize cDNA. qPCR targeting the N gene was then carried out using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) with the following primers: For: 5’-GCCTATGCCAACCCAGAATTA-3’ and Rev: 5’-CTTAACTACGAGCCCACCATAC-3’. ..

    Article Title: Anti-Tumor Effects of Cecropin A and Drosocin Incorporated into Macrophage-like Cells Against Hematopoietic Tumors in Drosophila mxc Mutants
    Article Snippet: Total RNA was extracted from 14 to 18 pairs of FB of mature third-instar larvae using Trizol Regent ® (Invitrogen, Waltham, MA, USA). .. After treatment with DNase I (Epicentre Technologies, Madison, WI, USA) to remove mixed genomic DNA, the purity of the RNA was checked by ensuring that the A260–A280 ratio of each RNA sample was between 1.8 and 2.0. cDNA was synthesized from the total RNA using a PrimeScript High-Fidelity RT-PCR Kit (TaKaRa, Clontech Laboratories, Shiga, Japan). .. Real-time PCR reactions were performed on a Thermal Cycler Dice ® Real-Time System III (TaKaRa Bio, Shiga, Japan) using TB Green ® Premix Ex TaqTM II Tli RNaseH Plus (TaKaRa Bio, Shiga, Japan).

    Article Title: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux
    Article Snippet: Then, migratory cells to the lower surface were fixed and stained with crystal violet (Sigma-Aldrich) for 15 min. After wash, cells were air dried and imaged. .. RNA was isolated from PASMCs and rat pulmonary arteries using E.Z.N.A.® Total RNA Kit I (Omega Bio-tek, Norcross, GA, USA) following the manual and reversely transcribed into cDNA with PrimeScript High Fidelity RT-PCR Kit (Takara, Shiga, Japan). ..

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..

    other:

    Article Title: MARCH8 Restricts RSV Replication by Promoting Cellular Apoptosis Through Ubiquitin-Mediated Proteolysis of Viral SH Protein
    Article Snippet: Total cellular RNA was extracted using the MagExtractor-RNA- (Toyobo, Osaka, Japan, Cat. No. NPK-201F) according to the manufacturer’s protocol. cDNA was synthesized using the PrimeScriptTM High Fidelity RT-PCR Kit (TaKaRa, San Jose, CA, USA, Cat. No. R022A) using oligo-dT primers.

    Article Title: OCT4 promotes lung cancer progression through upregulation of VEGF-correlated chemokine-1
    Article Snippet: To synthesize human VCC-1 cDNA, total RNA was isolated from A549 cells using a QuickGene RNA Cultured Cell Kit (AutoGene, Holliston, MA, USA) and used for cDNA synthesis using PrimeScriptTM High Fidelity RT-PCR Kit (Takara Bio, Otsu, Japan) according to the manufacturer's instruction.

    Purification:

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. After viral RNA purification, cDNA from viral mRNA was synthesized by reverse transcription reaction with oligo dT primer using PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio, Cat. #R022A). .. The amount of N mRNA was quantified by qPCR using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Cat. # QPX-201).

    Reverse Transcription:

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. After viral RNA purification, cDNA from viral mRNA was synthesized by reverse transcription reaction with oligo dT primer using PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio, Cat. #R022A). .. The amount of N mRNA was quantified by qPCR using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO, Cat. # QPX-201).

    Real-time Polymerase Chain Reaction:

    Article Title: Paramyxovirus matrix protein redirects METTL3 for dual regulation of viral replication and immune evasion
    Article Snippet: .. RT-PCR was performed with the PrimeScript High Fidelity RT-PCR Kit (TaKaRa Bio) using these strand-specific primers to synthesize cDNA. qPCR targeting the N gene was then carried out using THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) with the following primers: For: 5’-GCCTATGCCAACCCAGAATTA-3’ and Rev: 5’-CTTAACTACGAGCCCACCATAC-3’. ..

    Isolation:

    Article Title: Hypoxia-induced TPC2 transcription and glycosylation aggravates pulmonary arterial hypertension by blocking autophagy flux
    Article Snippet: Then, migratory cells to the lower surface were fixed and stained with crystal violet (Sigma-Aldrich) for 15 min. After wash, cells were air dried and imaged. .. RNA was isolated from PASMCs and rat pulmonary arteries using E.Z.N.A.® Total RNA Kit I (Omega Bio-tek, Norcross, GA, USA) following the manual and reversely transcribed into cDNA with PrimeScript High Fidelity RT-PCR Kit (Takara, Shiga, Japan). ..

    Cloning:

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..

    Construct:

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..

    Transfection:

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..

    Expressing:

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..

    Polymerase Chain Reaction:

    Article Title: O-GlcNAcylation of the intellectual disability protein DDX3X exerts proteostatic cell cycle control.
    Article Snippet: .. Cloning of DDX3X and OGT constructs for HEK293T transfection and E. coli expression A PCR product containing the Human ORF for DDX3X was obtained directly from RNA using the Primescript High Fidelity RT-PCR kit from Takara. ..



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